Ginsenoside Rb1 Alleviates Lipopolysaccharide-Induced Inflammatory Injury by Downregulating miR-222 in WI-38 Cells

Pneumonia is a serious respiratory tract infection disease in children, which threatens to the health or life of children patients. Ginsenoside Rb1 (Rb1) principle active ingredient extracted from root Panax notoginseng (Burk.) F.H. Chen with anti-inflammatory effect. Our study aimed determine effects and molecular mechanisms on lipopolysaccharide (LPS)-induced inflammatory injury lung fibroblasts WI-38 cells. Cell viability apoptosis were evaluated by CCK-8 flow cytometry, respectively. The production cytokines measured ELISA RT-qPCR. miR-222 expression was examined levels nuclear factor-kappa B (NF-?B) p65 phosphorylated detected western blot. We found that LPS stimulation induced cell inhibiting viability, inducing cytokine production, while treatment significantly attenuated LPS-induced Additionally, decreased upregulation activation NF-?B pathway Overexpression abolished inhibitory reduction, apoptosis, pathway. In conclusion, alleviated cells via downregulating inactivation NF-kB